Journal: Mediators of Inflammation

Article Title: The Vascular Endothelial Growth Factors-Expressing Character of Mesenchymal Stem Cells Plays a Positive Role in Treatment of Acute Lung Injury In Vivo

doi: 10.1155/2016/2347938

Figure Lengend Snippet: Assessment of lentiviral vectors mediated VEGF gene knockdown. (a) Assessment of the efficiency of lentivirus transduction using fluorescence microscopy, ×400. (b) The quantitative analysis for lentiviral transduction. (c) Evaluation of VEGF mRNA expression in the MSC, MSC-GFP, and MSC-ShVEGF groups. (d) Detection of VEGF protein levels in the culture medium of the MSC, MSC-GFP, and MSC-ShVEGF groups, respectively. (e) Image for the measurement of VEGF protein expression in the MSC, MSC-GFP, and MSC-ShVEGF groups by western blotting analysis. (f) The quantitative result for western blotting analysis (green, GFP; n = 3; # p < 0.05 versus MSC group and & p < 0.05 versus MSC-GFP group).

Article Snippet: Lentivirus carrying either GFP (LV3-GFP) or both GFP and ShRNA VEGF (LV3-ShRNA VEGF) was offered by GenePharma (Shanghai, China).

Techniques: Knockdown, Transduction, Fluorescence, Microscopy, Expressing, Western Blot

Journal: Mediators of Inflammation

Article Title: The Vascular Endothelial Growth Factors-Expressing Character of Mesenchymal Stem Cells Plays a Positive Role in Treatment of Acute Lung Injury In Vivo

doi: 10.1155/2016/2347938

Figure Lengend Snippet: VEGF expression in the LPS injured lung after the MSC, MSC-GFP, or MSC-ShVEGF treatment. VEGF protein levels in the LPS injured lung from different groups at 1, 6, and 24 hours after cells delivery measured by ELISA ( n = 6; ∗ p < 0.05 versus control group, # p < 0.05 versus ALI + PBS group, and & p < 0.05 versus ALI + MSC group).

Article Snippet: Lentivirus carrying either GFP (LV3-GFP) or both GFP and ShRNA VEGF (LV3-ShRNA VEGF) was offered by GenePharma (Shanghai, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control